Srikanth Konde

6 Questions 16 Answers 0 Followers

Questions related from Srikanth Konde

Is there any possibility of variation in PI value of a protein with theoritical value and actual value?

my protein is cry1 ac mol.wt 133.33 but it is precipitating below pH value @8.0

01 January 2017 3,547 3 View

Is there any possibility to give negative results in ELISA? If my protein present in Urea solution

My protein present in 8M Urea, 1mM DTT, 1mM EDTA, 1mM PMSF, 10mM Nacl & 50mM Tris @pH 8.0 And it is buffer exchanged with 50mM Tris, 10mM Nacl, 1mM DTT,EDTA & PMSF @pH 8.0

01 January 2017 4,513 3 View

Does the urea contain conductivity?

Solubilization buffer 2M Urea, 1mM DTT, 1mM EDTA & 10mM Tris.

01 January 2017 3,933 4 View

How can i purify my protein?

my protein PI is 5.0, with no Tag and mol.wt is 133kDa

12 December 2016 4,227 0 View

Is there any possibility of degradation of protein in 2M Urea?

My protein is recombinant one

12 December 2016 3,318 7 View

Sir/Madam please suggest me a proper protocol?

My protein is Cry1AC Mol.Wt 133kDa pI 5.0 (THEORITICAL) Protein Inclusion Bodies are forming. Please suggest me the buffers which can i use to 1. Cell Lysis (E.Coli, BL21DE3) 2. I.B Washes 3. I.B...

01 January 1970 3,528 4 View