7 Questions 23 Answers 0 Followers
Questions related from Tu Trinh
Dear all, We would like to check the purity of a GST-tagged recombinant protein by SDS-PAGE. We purified the protein using Baculovirus expression system. We run the reducing SDS-PAGE (GST was...
12 December 2016 7,883 10 View
Dear all, I'm checking the effects of the combination between two compounds in an asthma mouse model. During metabolism, these two compounds shared some similar cytochromes, such as CYP2C9,...
07 July 2016 9,713 3 View
Dear all, I would like to ask for your advice with culturing culture Eol-1 cells (eosinophilic cell line). I resuscitated cells from frozen stock and suspended in RMPI 1640+ 10% FBS + 1% PS. After...
05 May 2016 5,952 9 View
I used OneComp eBeads (eBioscience) to set up the compensation for PE & PE-Cy7. However, in the single PE staining tube, I couldn't achieve two distinct populations with similar mean values. I...
10 October 2015 204 7 View
Dear all, I am culturing EOL-1 cell line (a human eosinophil cell line) and I am trying to induce the activation of EOL-1 cells to release ECP by using Leukotriene E4 (300nM). Cells were seeded...
07 July 2015 3,341 2 View
I need to gate eosinophil on FACS by using double markers CDw125 (surface markers) and EPX (intracellular proteins). Here I already had PE antiCDw125, but for EPX, I need to do indirect staining....
02 February 2015 9,131 8 View
Dear all, I'm trying to detect protein which is located on mice cell surface by Immunocytochemistry. After collecting BAL cells, what is the best way to fix cells on slide without losing lots of...
10 October 2014 486 15 View