How can I study the expression dynamics of Alternative Lengthening of Telomere (ALT) associated proteins e.g. TRF1, TRF2, POT1 etc. in a cell line?

Chantal Agbemabiese @Chantal_Agbemabiese

10 October 2013 0 8K Report

PBMC isolated from whole blood samples from individuals confirmed to have chromosomally integrated Human Herpesvirus 6 (ciHHV-6) will be co-infected with Chlamydia trachomatis and the ALT associated proteins need to be studied. Any ideas?

Badges
Science topic

More Chantal Agbemabiese's questions See All

Does anyone know any online database which has all organisms classified based on whether they are intracellular or extracellular organisms?

I need to carry out classification of some bacterial pathogens based on whether they are extra- or intra- cellular.

06 July 2016 3,353 4 View

Can anyone help with analysis using TreeMap or Dendroscope to construct Tanglegrams?

I need some assistance to address an issue concerning host switch events.

09 October 2015 4,062 1 View

Is the term 'time to the most recent common ancestor' the same as 'time of the most recent common ancestor'? Can they be used interchangeably?

I was just wondering if both refer to the same concept and can be used interchangeably.

09 October 2015 6,516 0 View

What does the 't' or 'T' stand for in 'tMCRA' or 'TMRCA'? Do they represent the same thing?

I have come across tMRCA and TMRCA, I have also come across expressions like: 'time of tMRCA'. When I enquired about 'time of tMRCA', I was told it meant 'time of the Most Recent Common Ancestor'...

09 October 2015 8,798 0 View

How can we achieve better peace and security in the world?

What are some of the innovative and practical ways the world as whole can achieve peace and security aside the steps the UN has undertaken over the past years after the second world war? It seems...

01 February 2015 8,950 2 View

How do I get the age range (95% HPD) displayed at each node of an MCC tree in Figtree?

I have a maximum clade credibility tree drawn and I am trying to get the age range at each node but I don't seem to get it right. How do I get that? I have the node bars displayed but I need the...

11 December 2014 4,837 8 View

Can anyone suggest any other software apart from MEGA6 which I can use to draw a maximum likelihood tree?

The process is slow in MEGA so I was wondering if there is a software that is faster.

07 August 2014 9,977 5 View

Does anyone have an idea about how I can determine whether a gene reassortment event occured in the recent or distant past?

I want to be able to tell when a gene reassortment event (observed after DNA sequencing of genes of pathogens) occured.

05 June 2014 4,499 2 View

What is the simplest, user friendly but very reliable gene recombination detection software?

I need to determine whether any recombination events have occurred in genes I have sequenced.

05 June 2014 2,652 2 View

How reliable is the MAFFT online sequence multiple alignment program?

I have lots of sequences to align and I used to use MEGA6 but it takes forever to finish one multiple alignment session. I need a faster and a more accurate way of obtaining my multiple alignments.

05 June 2014 8,009 6 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View