Is there a German version of the WHO Workplace Violence in the Health Sector Questionnaire?

We are currently working on a project on health promotion of employees in the home care sector. To asess the current burden of this specific group, we would like to measure the degree of workplace...

29 June 2020 7,752 2 View

How to use dummy independent variables with multinomial logistic regression?

I am using a multinomial logisitc regression in Stata. I want to include 75 districts as a possible explanatory variable, especially to check region effect. Could anyone please help me how to...

09 August 2019 5,597 22 View

How to measure centralisation of Human Resource practices?

Hi. My research topic is Centralisation versus decentralisation of HR practices. Independent variable of my research question is the level of centralisation and dependent is recruitment and...

15 October 2018 2,216 7 View

CFX96 single tubes with optical cap?

17 March 2016 8,822 3 View

How can we set a prior for scale parameter in the case of compound weibull distribution with two shape parameters?

Optimal bayesian parameter estimation of compound weibull distribution. Any good suggestion please?

23 June 2014 7,306 3 View

How can we use PCAs to compare two groups?

I want to compare a survey data for two groups using their corresponding PCAs but I have no clue that how can we use PCAs for comparison.

07 June 2014 4,126 6 View

Is it possible to generate nanowire through sol-gel method?

Sol-gel technique is prominent method used globally to prepare nano-particles.

25 May 2014 2,357 3 View

Should we change the grading system from grades to pass/fail only?

Should we change the grading system from grades to pass/fail only?

01 January 1970 1,875 29 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View