Hi,
I was wondering if anyone using the TWIST NGS Methylation protocol has experienced issues with under-conversion of CpGs? Thanks.
I actually need a straight forward downloadable zip file of images. but whatever i found on google takes much process ( gives me contract pdf to sign and send them, dont know they even reply or...
09 October 2020 147 3 View
We are currently working on a project on health promotion of employees in the home care sector. To asess the current burden of this specific group, we would like to measure the degree of workplace...
29 June 2020 7,752 2 View
I am using a multinomial logisitc regression in Stata. I want to include 75 districts as a possible explanatory variable, especially to check region effect. Could anyone please help me how to...
09 August 2019 5,597 22 View
Hi. My research topic is Centralisation versus decentralisation of HR practices. Independent variable of my research question is the level of centralisation and dependent is recruitment and...
15 October 2018 2,216 7 View
I am quantifying amoA genes (bacteria) in soils samples. I heard that these genes have more than one copy in single genome. So I would like to ask that is there any relevant literature available...
11 January 2018 1,312 3 View
17 March 2016 8,822 3 View
06 August 2015 9,474 1 View
I am working on fabrication of CVD graphene FET. I am using PPR S1813 photoresist for lithographic process. I want to know different methods by which PPR s1813 residues can be removed from...
13 February 2015 4,304 10 View
I have been working on exfoliated graphene FET devices. When I fabricate devices and characterize them, dirac point shifts to very high voltages e.g., 80V. I have tried chloroform cleaning after...
31 January 2015 3,029 9 View
Is it necessary to keep subject under monochromatic light or sodium vapour lamp if administered with mesalamine? what will be impact on sabject safety or study data if blood sample collection is...
05 July 2014 5,090 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...
24 February 2021 8,856 3 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View
Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks
23 February 2021 6,518 3 View
I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...
19 February 2021 7,006 5 View
Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...
18 February 2021 8,719 1 View
I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...
17 February 2021 7,575 2 View