Hi,
I was wondering if anyone using the TWIST NGS Methylation protocol has experienced issues with under-conversion of CpGs? Thanks.
Hi, Attempted to extract cfDNA from a 4 mL plasma sample, with poor yield. Went through the manual in the section regarding poor yield, and feel that this does not apply. Some potential...
14 July 2024 7,475 1 View
Hello, I am currently conducting research on ovarian tumour cells and the use of chemotherapies with dietary metabolites and their combinations to see if there is a difference in cell viability. I...
01 July 2024 6,099 2 View
We want to clean manually (and regularly) the optical sensor of our device. We know it is a delicate piece of equipment so we are looking for practical indications, video, or any type of help.
03 April 2024 5,117 1 View
Being a college professor i want to have a research paper on skill gap analysis in an industry
11 March 2024 259 0 View
I am searching for ideas, examples, research papers, projects etc, etc. regarding possibilities to implement behavior analysis in caring/nursing science/practice. My best regards, Sabina Dalsborn
24 February 2024 4,191 13 View
One of my research paper cited 2 times but on research gate it shows 1 citation Please check the reference cited as Pathan NP, Sisodiya DB & Raghunandan BL (2023): First report of stem fly...
03 November 2023 6,876 2 View
Hi, I am doing my dissertation as a systematic review and meta-analysis on the efficacy of daratumumab in relapsed and refractory multiple myeloma. I am using clinical trials as my source and do...
16 April 2023 7,608 2 View
I am trying to get insights on S.sidera mycobiome and I was using ITS-1F and ITS-2R primers to do so, however, I think most of my reads correspond to a coral, could that be possible?
14 February 2023 7,281 0 View
I want to add these compounds to my cell culture. Does anyone have any experience in what is the best solvent to dissolve these compounds? I am planning to dissolve NE and MCOPPB in water and...
06 February 2023 2,417 0 View
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11 January 2023 5,918 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,136 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,078 0 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.
28 July 2024 734 0 View
Gene sequencing related trouble shooting
25 July 2024 4,147 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,075 3 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,404 2 View
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
18 July 2024 1,576 2 View
I am conducting free radical polymerization using Kraft lignin and MMA, with DMSO serving as the solvent and there is also an initiator. Following the reaction, I obtain a solution, but I am...
15 July 2024 9,826 1 View