In the lab we use a semi-dry apparatus for protein transfer (it is a T70 from Amersham). The cover electrode gets more and more dirty due to salts and maybe limestone that deposit, even if we rinse with distilled water after each use.
Do you have any recommendations to remove these particles? Do you think we can rinse the electrode with acetic acid for example?
Hi Pauline,
in our lab we use MilliQ water and a 20% Methanol/10% acetic acid in MilliQ water (Destain for coomassie gels) for the cleaning of our cover electrode. It works pretty well, as long as you use it every now and then.
All the best,
Kai
Hi Pauline. Another prossibility is to use nitric acid (1N) to clear the cover electrode. Just put it on some paper, and clean with it and then wash with ddH2O. hope also this work.
I contacted GE Healthcare to have their tips. After removing the electrode, I washed it 1st with warm deionized water to remove particles such as glycine. Then I washed it in 20% acetic acid. All the particle were limestone that disappeared !
So I skip the 3rd advise, that was to wash in 1M sulfuric acid to remove metals (that can be contaminants in buffer or methanol). After rincing with deionized water, I can used my transfer machine again!
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