Hi Pauline,

I performed western blot with primary Ab anti-phosphoXPA (O/N incubation) , secondary anti-rabbit HRP-conjugated and I was not satisfied with the results. When I increased contrast and brightness on LICOR scanner, the signal was very weak and I could see lines on membrane which corresponded to lines of holding frame. However, reason for this could be that low affinity of my primary Ab to target protein or low protein load on membrane led to higher contrast and brightness needed and to artifacts masking my signal.