I isolated a human genome through the CTAB method, the band is good enough but amazingly 260/280 ratio is more than 6.Please suggest what the contamination could be and how I can remove it by simple a manual method?
The result is really amazing. I never encountered such high values at 260/280 ration. I received maximun of 2 during my research following the CTAB method.
Regarding your query about how to remove RNA contamination, you can use RNAse A enzyme (at 10mg/ml) followed by P:C:I treatment.
Dear Ghulam,
Arvind is rite, you can use RNase A to remove RNA contamination.
Thanks to provide me such supportive answers... but i need more cheaper method... and hopefully Mr. Artur Burzynski have right guess... I will repeat other method to find concentration beside spectrophotometer.
I don't think that is RNA contamination, which should also be around 2. You can use phenol chloroform extraction method to purify your RNA again. The method is very easy and cheap. You can find protocol here http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/9/77619dat.pdf.
I agree with the other comments here. It is not terribly expensive to retreat your samples with RNaseA and then purify using Phenol chloroform isoamylacetate ph8.1. Additionally you could use a PCR product cleanup kit like one from Qiagen and run your samples through columns.
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