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We have planned to sequence a PCR product at MiSeq platform. after 1st step of PCR we perform clean up step. The yield after clean up is < or = to 1ng/ul. Kindly add your advice in respect that...
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I have seen in lab that my colleagues used different concentration of NaCl for CTAB preparation as per their tissue requirement. Is there any body who can update me on the required concentration...
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I isolated a human genome through the CTAB method, the band is good enough but amazingly 260/280 ratio is more than 6.Please suggest what the contamination could be and how I can remove it by...
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