How can I remove DNAk from my protein solution?

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Mohammed Milhim

If you add ATP to your washing buffer during purification you will be able to release the protein from the Dnak.

Your protein will not be affecting because it is already folded.

Dominique Liger

Hi there,

DnaK/ADP exhibit the highest affinity towards protein. By proposing ATP in large excess, exchange ADP/ATP should occur and this exchange will result in a weakened DnaK/protein interaction.

David Vizarraga

I read that MgATP mM in a wash buffer It is enough for separated DNAk from my protein