Alessandro Pistone

4 Questions 2 Answers 0 Followers

Questions related from Alessandro Pistone

How can I induce mitochondrial membrane decoupling in neuronal cells SH-SY5Y?

Good morning. I am working on cell cultures of neuroblastoma SHSY5Y and in order to evaluate the effect of a protein on the mitochondrial membrane, I thought about performing a functional test....

21 May 2025 5,696 0 View

How can I induce mitochondrial membrane decoupling in neuronal cells SH-SY5Y?

Good morning. I am working on cell cultures of neuroblastoma SHSY5Y and in order to evaluate the effect of a protein on the mitochondrial membrane, I thought about performing a functional test....

21 May 2025 4,192 0 View

How can I remove albumin from Human serum?

I have applied a general protocol. In short: 20uL of serum were added to 8 volumes of a cold TCA+ Acetone solution. After a light stirring I incubated for 90 minutes at -20 followed by centrifuge...

27 September 2024 5,388 3 View

How can I remove immunoglobulin from small volumes of serum?

Good morning, working on patient serums, I noticed that in western blotting tests the IGs give false positives and unspecific signals. Ask if anyone can give me a methodology to remove these...

10 August 2024 597 3 View