How can avoid penetration of contact surfaces in ansys apdl, Contacts were created in Hypermesh.

When we twist a bar inside a slot bar had contact on slot walls. Problem is bar is penetrating inside the wall. How to avoid. Contacts were made in Hypermesh, analysis done in ansys apdl.

09 September 2016 2,545 3 View

Why am I getting deflection after applied zero force in ansys transient analysis during elastic stage instead of zero deflection?

I did multiple load steps with ansys transient, after some time when i applied zero force, my model gives some deflection, but within elastic limit, i think that deflection (DMX) should be zero,...

21 September 2015 8,872 6 View

Can you suggest how to find a model is subjected to plastic deformation based on ansys results?

17 June 2015 7,421 4 View

How do you draw a theoretical XRD pattern using cell parameter or experimental data?

See above

22 July 2014 6,339 27 View

How to create multiple load step in ansys for a solid model?

Time varying load step.

02 June 2014 7,934 8 View

Does a space group change via centering translation will give an additional peak in the XRD?

Centering translation does not reduce the no of symmetry elements. only position of symmetry elements varies Ex P63 /mmc to P63 /mcm.

16 July 2013 5,119 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View