How can I proliferate HCV in cell lines by the feasible technique?

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How to mesh without obsolete mesh of a skull with teeth, bones, bracket and archwire assembly? Also how to do convergence of this? Ansys workbench?

Have to do perfect error free mesh of a maxilla with cortical, cancellous, orthodontic brackets, archwire etc with contact in Ansys workbench. How to check mesh quality with convergence as well.

28 February 2024 7,266 0 View

Anybody suggest Pan-HCV and Pan-HBV for PCR control purpose?

Hi to all, Does anybody suggest Pan-HCV and Pan-HBV for conventional and real-time PCR-positive control purposes? Due to viral instability, we are unable to use the known positive samples for...

06 February 2024 4,362 3 View

Any correlation between HBeAg and HBV PCR, is this reciprocal?

Statement 1: we got one sample to have HBeAg ELISA and HBV PCR both Positive, indicating active replication present in the HBV virus. Statement 2: we got one sample to have HBeAg ELISA negative,...

06 February 2024 1,594 2 View

What is the crop coefficient of high yielding coconut palms at spacing of 7.6X7.6 m?

Need the crop coefficient of coconut palms to arrive at the irrigation water (ETc)requirement. Does the irrigation water quality affect the crop coeffecient of a crop.

07 May 2020 8,142 0 View

Will coconut palms grow in highly saline coastal soils ?. What is the maximum soil EC coconut palms tolerate?

Need to understand if coconut palms can be grown in coastal soils with pH 7.5 and salinity ranging from 86 to 140 ds/m and high calcium carbonate content?

07 May 2020 3,246 0 View

Principal Componenet Analysis for 3D matrix code in java?

I am having Hyperspectral data. 10 x 10 x 2151(x,y,z axis).For this i have to do PCA, in that process i have to reduce Z axis (2151) in java code.any code available pls suggest

21 November 2019 932 0 View

How to mark tiny (0.001 mm dot on stainless steel) dot on steel?

I have to mark very very small (0.001 mm) permanent dot on steel specimen as a reference point.

13 May 2018 1,071 6 View

How can avoid penetration of contact surfaces in ansys apdl, Contacts were created in Hypermesh.

When we twist a bar inside a slot bar had contact on slot walls. Problem is bar is penetrating inside the wall. How to avoid. Contacts were made in Hypermesh, analysis done in ansys apdl.

09 September 2016 2,589 3 View

Why am I getting deflection after applied zero force in ansys transient analysis during elastic stage instead of zero deflection?

I did multiple load steps with ansys transient, after some time when i applied zero force, my model gives some deflection, but within elastic limit, i think that deflection (DMX) should be zero,...

21 September 2015 8,903 6 View

Can you suggest how to find a model is subjected to plastic deformation based on ansys results?

I have ansys results of my model. I wish to know whether plastic deformation happens in the model due to force.

17 June 2015 7,460 4 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Sandip Chakraborty

You can refer to the following links to get certain valuable details:-

1. http://dspace.nbuv.gov.ua/bitstream/handle/123456789/5637/05-Rybalko.pdf?sequence=1

2. www.nature.com/onc/journal/v33/n22/extref/onc2013225x7.doc

Ishtiaq Qadri

Certain cell lines like Huh7 can support the HCV infection in vitro for few days and can be used for antiviral drug testing

Magesh Sachidanandam

Actually we have the Huh7 cell lines, but it can support the HCV infection in vitro by transfected with cloning vectors only.