Anybody suggest Pan-HCV and Pan-HBV for PCR control purpose?

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Aarya Chitransh

For the purpose of positive controls in conventional and real-time PCR for Hepatitis C virus (HCV) and Hepatitis B virus (HBV), there are commercial options available. You can consider the following:

Commercial kits: Various biotechnology companies offer positive control materials specifically designed for PCR assays targeting HCV and HBV. These kits typically contain synthesized DNA or RNA fragments that mimic specific regions of the viral genome, serving as positive controls for PCR amplification. Some well-known companies in the field of molecular diagnostics and PCR kits include Thermo Fisher Scientific, Qiagen, Bio-Rad, and Takara Bio, among others. You can explore their product catalogs or contact their customer support for specific kits tailored to your needs.

Reference articles: While there may not be specific articles recommending commercial products for positive controls in PCR assays for HCV and HBV, you can search for research papers or reviews on the development and validation of PCR assays for these viruses. These articles may provide insights into the selection of appropriate positive controls and reference materials. Additionally, you can refer to guidelines or protocols published by organizations such as the World Health Organization (WHO) or the Centers for Disease Control and Prevention (CDC) for recommendations on PCR assay validation and quality control measures for viral detection.

When choosing a commercial kit or reference material, ensure that it meets your specific requirements in terms of target sequences, assay sensitivity, stability, and compatibility with your PCR protocols. Additionally, consider factors such as cost, availability, and regulatory compliance in your decision-making process.

Veenavadhini Ramalingam

Hello,

Have you ever tried buying NIBSC standards that you can use as positive controls which is very stable and also it comes as a lyophilized powder?

HI,

Well, Thermo scientific has a positive control which I guess you can use for your experiment.

(Acrometrix BB NAT HBV positive control.)