I need help for image analysis for spot detection for gel excision.
I have my work on a particular topic and I would like to share it with Research Gate. Show me please, how to do it.
17 October 2023 2,180 4 View
My paper is uploaded but the topic is not agree with my research paper. I don't know why?
16 September 2023 342 2 View
I am a master's student of environmental science and trying to calculate the GHG emissions from electricity generation industries using the tier 1 method of IPCC guideline and LEAP model. When I...
20 July 2023 864 3 View
I am conducting Avian Influenza virus detection by real time rt PCR. I did 4 target AI genes and 4 primer probe sets for PCR. (H7 HA, H5 HA, NP and M target gene) I run real time 4 time...
04 April 2023 9,444 1 View
I have both forward, reverse and probe sequences and I want to calculate accurate base pair length to check band for PCR products. (Forward primer)nnnn(Reverseprimmer) is not work in blast. I...
09 March 2023 8,678 3 View
Dear all, I would like to know the expect image of DNA under SEM microscope. How can we see DNA under microscope? I used the sample of pure nucleic acid and viewed under SEM and I got this pic....
07 February 2023 653 2 View
I am doing fourplex mqPCR panel for pathogens by Taqman probe . I did well choose for different dyes option (reporting dyes). real time PCR is five channel 7500 applied biosystem. For primary...
11 December 2022 840 4 View
No affiliated organisation to attach.
22 March 2022 3,442 1 View
I am a 4th year Civil Engineering student and am from Philippines. We have a problem with traffic and floods. Is there anything you can suggest to be a good undergraduate research topic related to...
28 August 2021 2,156 6 View
I am doing critical appraisal and using JBI appraisal tool. I m not quite sure how to critique this paper due to unclear study design My understanding is Retrospective can only be observational...
15 May 2021 7,998 3 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View
I fabricated Ti3C2Tx using concentrated HF 40%, I plot an XRD as attached image below.. please let to know if I obtained it or not.
02 August 2024 6,789 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...
29 July 2024 2,229 0 View
