How can I prepare a Stock and Work Solution of Acridine Orange Dye (for Integrity of DNA) and PNA-Alexa 488 (for acrosomal integrity)?

More Camilo Hernández-Avíles's questions See All

Which air pollution/air quality books do you recommend for working with environmental engineering students?

I am exploring up-to-date books on air pollution and air quality, and I would like to know which books do you recommend for environmental engineering students.

25 January 2021 3,972 7 View

Numerical taxonomy with SPSS?

Hi everyone, I was wondering about the possibilities of performing numerical taxonomy with SPSS software. I would be very thankful to recieve advice!! For now I have been reading about...

12 January 2021 5,549 3 View

I would like. How they can be simulated the deformational processes of valvular aortic stents??

We have a projet about TAVI

22 December 2020 7,729 1 View

Using multiple linear regression to solve a multiple-way ANOVA problem?

Specifically, I want to know if I can use multiple linear regression to predict a response value 'y' from it's associated "treatment" or "group" averages in several variables. Let's say:...

04 November 2020 632 19 View

PRECIPITATION MODELING OF V CARBIDE IN Mn STEEL?

HELLO DEAR COLLEGUES, Is there anybody who have acces to MATCALC software that help me modelling the precipitation phenomena in a medium Mn steel microalloyed with 0.15%wt. of V? The expected...

05 October 2020 5,914 3 View

How to quantify relative amounts of a substance with immunostainings through confocal microscopy when samples have high autofluorescence?

I am working with plant tissues, buds specifically, fixed in glutaraldehyde and embedded in LR White resin. I am working with the primary antibodies LM19 and LM20 targeting pectins. I am using a...

21 September 2020 5,213 6 View

Does the molecular weight of PVP matter in CTAB buffer for DNA and RNA extraction?

I am preparing CTAB buffer and I read that addition of PVP (Polyvinylpyrrolidone) helps to remove phenolic compounds. Most of the times in protocols there is no additional information about...

05 September 2020 7,488 4 View

Why does the 2D peak intensity in a graphene Raman spectrumdecrease with the number of layers?

If we compare a Raman spectrum of monolayer graphene and bilayer or fewlayer graphene, we will see that the 2D peak intensity decreases (relative to the G peak intensity) for the later cases. I...

24 July 2020 907 3 View

One-way ANOVa for non-homogeneous and not normal data?

I have a data set that has 3 groups and a single variable. Under normal circumstances, a one-way ANOVA would be used for comparisons between my three groups. HOWEVER I ran a Shapiro-Wilk test for...

22 July 2020 6,061 7 View

In which solvent should I reconstitute mitoTEMPO?

Dear colleagues, I was wondering which solvent should I use to reconstitute mitoTEMPO, I've seen I could use water or DMSO but I'm not sure about the stability of the compounds in the respective...

18 May 2020 2,882 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Matthias Krienke

Http://edoc.ub.uni-muenchen.de/1338/

it is the same for bulls and stallions sperm but the use of syto 17 is a fault sorry. You have to leave the living sperm unstained or you had use a red laser for syto 17.

Good luck

(but please think at the few DFI-sperms by bull sperma (0-3%) stallion sperms like human sperms the cut off is arround 10%)