How can I prepare a Stock and Work Solution of Acridine Orange Dye (for Integrity of DNA) and PNA-Alexa 488 (for acrosomal integrity)?

More Camilo Hernández-Avíles's questions See All

Which air pollution/air quality books do you recommend for working with environmental engineering students?

I am exploring up-to-date books on air pollution and air quality, and I would like to know which books do you recommend for environmental engineering students.

25 January 2021 3,972 7 View

Numerical taxonomy with SPSS?

Hi everyone, I was wondering about the possibilities of performing numerical taxonomy with SPSS software. I would be very thankful to recieve advice!! For now I have been reading about...

12 January 2021 5,549 3 View

I would like. How they can be simulated the deformational processes of valvular aortic stents??

We have a projet about TAVI

22 December 2020 7,729 1 View

Using multiple linear regression to solve a multiple-way ANOVA problem?

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04 November 2020 632 19 View

PRECIPITATION MODELING OF V CARBIDE IN Mn STEEL?

HELLO DEAR COLLEGUES, Is there anybody who have acces to MATCALC software that help me modelling the precipitation phenomena in a medium Mn steel microalloyed with 0.15%wt. of V? The expected...

05 October 2020 5,914 3 View

How to quantify relative amounts of a substance with immunostainings through confocal microscopy when samples have high autofluorescence?

I am working with plant tissues, buds specifically, fixed in glutaraldehyde and embedded in LR White resin. I am working with the primary antibodies LM19 and LM20 targeting pectins. I am using a...

21 September 2020 5,213 6 View

Does the molecular weight of PVP matter in CTAB buffer for DNA and RNA extraction?

I am preparing CTAB buffer and I read that addition of PVP (Polyvinylpyrrolidone) helps to remove phenolic compounds. Most of the times in protocols there is no additional information about...

05 September 2020 7,488 4 View

Why does the 2D peak intensity in a graphene Raman spectrumdecrease with the number of layers?

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24 July 2020 907 3 View

One-way ANOVa for non-homogeneous and not normal data?

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22 July 2020 6,061 7 View

In which solvent should I reconstitute mitoTEMPO?

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18 May 2020 2,882 3 View

How to compare two groups with only two measurements?

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03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

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03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

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03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

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03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

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03 March 2021 8,920 3 View

How to increase a model accuracy ?

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03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

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02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

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02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

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02 March 2021 752 2 View

Matthias Krienke

it is the same for bulls and stallions sperm but the use of syto 17 is a fault sorry. You have to leave the living sperm unstained or you had use a red laser for syto 17.

Good luck

(but please think at the few DFI-sperms by bull sperma (0-3%) stallion sperms like human sperms the cut off is arround 10%)