Camilo Hernández-Avíles

5 Questions 8 Answers 0 Followers

Questions related from Camilo Hernández-Avíles

Can somebody send me an example of an assay in which Viral Seroneutralization Assay was used instead ELISA?

I need a research paper in which Viral Seroneutralization Assay was used instead ELISA, and also reasons for this decision.

03 November 2014 7,070 7 View

How can I prepare a Stock and Work Solution of Acridine Orange Dye (for Integrity of DNA) and PNA-Alexa 488 (for acrosomal integrity)?

I have at my lab the reagents Acridine Orange (Sigma) and PNA-Alexa 488 (Molecular Probes), but I don't know how can I prepare the Stock and Work solutions of this reagents? Somebody has a working...

23 March 2014 6,572 2 View

How can I read the results of a FITC-PNA/PI, CTC, SCSA and SYBR-14/PI assays in mammalian sperm?

I want to develop fluorescent assays to evaluate the membrane integrity, capacitation status and acrosome reaction in bull and stallion semen. So, what are the possible results of this assay, and...

28 November 2013 5,894 4 View

Is there a relation between the components of the cooling/freezing extenders of equine semen and the posible result of a Hypoosmotic Swelling Test?

Is it possible that the sucrose included in the cooling/freezing extenders modifies the osmolarity of the HOS test?

13 November 2013 4,178 1 View

What's the mechanism for epinephrine-induced capacitation in bovine sperm under in vitro conditions?

Epinephrine induces calcium influx into the sperm, activating the metabolic pathways that lead to increased cAMP and subsequent hyperactivation, protein tyrosine phosphorylation and acrosome...

11 October 2013 3,220 8 View