If I do not use total protein normalization, is there any way to perform immunoblot normalization other than housekeeping proteins?....
For the protein extracts from food matrices, without any housekeeping genes/proteins how can I normalize and illustrate the fold change at my target protein using western?
Should I choose an internal protein roughly constant in the food matrix other than the application of GAPDH and/or β-actin...
Hi Ismail,
I suggest applying a proper external standard, e.g. a known protein behaving similarly to the protein of interest in the investigated food matrix. You might work out a protocol for adding the known protein before the extraction procedure, similar to the quantification of lipids in tissue samples when spiking the sample with known amount of standard.
Best, Karoly
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