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Questions related to Nanoparticle Research
i am doing Colocalization Studies i used Hochest 33342, cell mask and Lysotracker deep red, orange and red. please any one suggest me another lysotracker dye and lysosensore dye according this...
22 February 2024 8,249 0 View
Hi all, I'm working on MDCK II cell line where I grow the cells on PDMS substrate into a dense tissue (about 5mm diameter in size). I have been trying out different plasma membrane dyes for my...
15 January 2024 9,808 0 View
I want to prepare the stock for use in the FO system. I want to synthesize the pesticides.
24 October 2023 8,968 0 View
Which one is the correct way to practice? a). Dissolving 1000mg of Lead nitrate into 1L water; ==> Concentration = 1000mg/L as a lead ion. b). Follow the formula: Molecular weight of lead...
31 August 2023 5,170 0 View
Hi All, I am using cell mask orange dye to label HEK 293 T cells plasma membrane. I am using 5 ug of dye as final concentration and incubating cells for 10 min at 37•C. I am facing issue with dye...
31 January 2023 7,520 7 View
When we dope ZnO with Co or Fe or Ni its band gap mostly decreases, but sometimes it increases with it. What's the reason behind it?
17 September 2022 5,918 5 View
As ZnO has 60 meV exciton binding energy which we call as large exciton binding energy. So what is the range from which we can say that it is either maximum or minimum?
08 August 2022 2,119 1 View
I found out that likely factor, causing degradation of my RNAs during decapping with hDCP2, is non-enzymatic reaction caused by Tris-HCl and Mg2+. All alternative buffers I found contain both in...
06 April 2022 6,723 0 View
How to minimize the effect of impurities from surroundings supercell images on our model supercell? My concern is about the surrounding periodic supercell images where also the modeled impurity...
07 August 2021 1,267 5 View
I want to prepare a solution of humic acid with concentration 4mM and 100mM but i can't find it's molecular weight
08 March 2021 9,255 6 View
I have a sample of mixtures containing polystyrene microspheres (PS-MS) with 3 different diameter sizes (bought from thermo), they all contain a green fluorescent dye inside the polymer matrix /...
07 January 2021 2,348 5 View
I need to conjugate thiolated oligos to GNPs and I am wondering what concentrations of the oligo to use with what concentration of GNPs for effective conjugation.
09 December 2020 8,111 2 View
I am trying to make a Silver nanoparticle covered with FMN ligand. I tried docking using AutoDock but only a molecule can be docked on the nanoparticle but more than hundreds are required to cover...
09 October 2020 2,245 6 View
Hello, I am trying to form a pellet of PMMA particles. I have tried centrifuging for 1 hour at 26000 RPM (highest the centrifuge I have will go) but the particles do not form a pellet they...
10 September 2020 8,939 5 View
I want to show that my developed nanoparticle targets hypoxia but from doing research it seems like most hypoxic targeting related experiments are done in vivo. I'd like to be doing in-vitro...
17 August 2020 3,878 4 View
Hello! I am working on optical sensors to detect few gases at high temperature. Gold nanoparticles prepared using citrate method (with intense absorbance peak at 530nm) are drop casted on quartz...
29 July 2020 2,345 0 View
Is it possible to calculate the average size of osteoblast or osteosarcoma cell in cell culture medium using DLS?
14 July 2020 478 7 View
We are considering to buy GC/MS. Can experts out there share your review with your current GC model?
26 June 2020 6,295 21 View
I want to perform adsorption using DFT code but I can only create nanosheet like structure - supercells from primitive and use them to check their adsorbing selectivity towards certain gas. I...
07 June 2020 1,759 5 View
In many papers, it was given that limit of detection (LOD) = 3S/m that Please explain the terms in it. Could you please explain how to get these parameters from origin?
01 June 2020 8,546 1 View
I have done an experiment in which mercury engulfs silver and when we added sulfur it turns to black and after the incineration process at 650-degree celsius for 3 hours, we obtained a black color...
04 May 2020 4,972 11 View
As we know that some researcher are suggesting the diffusion of corona virus by nanomaterials. it may be that corona virus can be deactivated by spinel [AB2O4] nanoparticles. i want know some...
17 April 2020 7,498 6 View
I am currently trying to encapsulate a peptide into PLGA nanoparticles and use Poly(ethyl methacrylate) (PEMA) to coat the surface to add more carboxylic groups. However, I am having a hard time...
24 February 2020 9,280 3 View
I was trying to design a nanocluster of 10 nm diameter using Material studio(MS) software. Due to the lack of the "nm" size option in the material studio, I have used a 50Å (angstrom) radius...
13 February 2020 4,820 5 View