4 g of air-dried soil sieved through 0.5 mm was weighed in 15 replicates and place in 50 mL centrifugation tubes numbered from 1 to 15. 10 mL of deionized water was added to all the samples. 0.5 mL of indifferent CaCl2 0.1 M solution. To the centrifugation tubes numbered 1 – 7, increasing quantities of 0.1 M HCl solution was added but to the centrifugation, tubes numbered 9 – 15 increasing quantities of 0.1 M NaOH solution was added. The volume of the mixture was top up to 20 mL with deionized water. Afterwards, the mixture was allowed to stand for 4 days with occasional agitations. The pH ®ΔpH 0.002 M was measured. 0.5 mL of 2 M CaCl2 was then added and subjected to a 3 hour shaken with the help of end – to – end mechanical shaker and the pH →ΔpH 0.05 M was measured using An electrometric pH meter was standardized using 2 buffer solutions (pH 4 and 7 for acidic soils or 7 and 10 for basic soils). DpH = (pH 0.05 M – pH 0.002 M) was calculated. A graph of DpH versus pH 0.002 M was plotted to determine the point where DpH = 0 which corresponds to pH0.
the results are not encouraging can someone suggest another method or a modified method that can measure the point of zero charge?
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