Hello,

I have some problems to record changes in intensities of GcAMP signal.

I inserted my GcAMP lenti viral vector in iPSC-derived cortical neurons. I tried to record the GcAMP signal in these cells through live cell imaging but the signal is very low and I cannot actually see any changes in intensities in time. I also tried a normal over expression, but I had the same problem.

Someone knows if I have to use some chemicals to induce the GcAMP signal?

Thanks,

Mara