What tests for: 1. differences between continuous variables when grouped with different categorical ones; 2. significance of potential relationships?

Hello. I was wondering if anyone had suggestions in terms of what exploratory analysis to carry out for my dissertation with the following data. I measured N=26 participants pre and post an...

09 August 2020 5,256 5 View

Anti-His Antibody binding everything on a western?

Hello all, I've been expressing some His-tagged proteins in E.coli that appears to be going just fine based on how the SDS-PAGE gels look, so I wanted to confirm with a Western. I transferred my...

22 July 2020 5,255 5 View

Is it possible to run a Mediation Analysis with Process when the dependant variable is a percentage value?

I measured the DV by presenting the participants two product options where they had to choose one of them. I repeated this 20 times. After that I calculated the percentage value of choosing the...

05 November 2019 9,978 3 View

How to codify a sequence DNA correctly for DNA barcoding analysis?

Hello, I am starting a new project with DNA barcoding and I am not sure how to codify sequence DNA. In this project we are using the following molecular markers: rps16-trnk, rpl32-trnl and...

26 July 2018 5,797 2 View

Transfection of S2 cells?

Does anyone work with expression in S2 cells? How efficient is the transfection using Lipofectamine 2000? Everytime I try to transfect using CaCl2 I get a cloudy media, and it seems that the...

30 June 2018 9,145 1 View

PCR using oligo dT?

Is it possible to perform a PCR, using as the reverse primer a oligo dT, the same used in the reverse trancription? I am trying to amplify a mRNA, but with no success

21 June 2018 3,707 3 View

Is it possible to impose a cpu limit on executors in Apache storm?

Each executor is a thread inside a worker process. Is it possible to set some rule such that all the executors on a machine execute for (roughly) equal time? so that one executor is not hogging...

12 February 2018 8,355 1 View

Denaturing RNA gel

Does anyone has a denaturing RNA gel protocol, by just adding guanidine to the sample, and running in a regular agarose gel?

19 July 2017 8,078 3 View

How do you do the normalisation of X protein to study the effect of a protein Y mutation on X protein level by western blot?

I need to study the effect of the protein Y mutation on the levels of a protein X. So I over-expressed the protein X under 5 conditions: (1) not over-expression of protein Y (2) with...

07 June 2017 5,252 4 View

Is it possible to load 120 ug of proteins in a protein gel?

I need to load 120 ug of proteins in a protein gel. Do I need some precaution for the protein migration and western-blot?

11 July 2016 624 4 View

What is the composition of TM buffer used for concentrating bacteriophage?

Hello everyone, I have to isolate DNA from Brucella phage , but before that, I have to concentrate the phage using PEG 8000. I have got protocol for concentrating the phage using PEG and TM buffer...

24 January 2021 2,767 6 View

Can anyone suggest a kit or protocol to extract Brucella spp. DNA from FFPE blocks?

Hi everyone! I am trying to extract Brucella DNA from FFPE blocks. Can anyone suggest me any good kit or protocol? I checked many articles and kits but still could not find an appropriate kit or...

15 December 2019 9,767 2 View

Can anyone suggest a kit or protocol to extract Brucella spp. DNA from FFPE blocks?

Hi everyone! I am trying to extract Brucella DNA from FFPE blocks. Can anyone suggest me any good kit or protocol? I checked many articles and kits but still could not find an appropriate kit or...

11 December 2019 6,223 2 View

What is the best way to detect the presence of a live attenuated vaccine in blood samples?

Hello! I want to know if there is a better way to detect the presence of a live attenuated vaccine in animal blood samples. We use the buffy coat fraction to extract the DNA and perform a PCR...

17 June 2019 7,018 8 View

What is the best way to detect the presence of a live attenuated vaccine in blood samples?

Hello! I want to know if there is a better way to detect the presence of a live attenuated vaccine in animal blood samples. We use the buffy coat fraction to extract the DNA and perform a PCR...

05 June 2019 8,471 10 View

Did any researcher designed primers for identification and differentiation of the three Brucella melitesnsis biovars 1,2 and 3 by PCR ?

Current primers designed to identify Brucella organisms on species levels, either abortus, suis, melitensis, ovis, neotoma, or recent brucella species as marine etc. . But there is no primers...

03 April 2019 5,052 2 View

Why brucellosis (Malta fever) is consider the main cause of abortion in infected animals while it is not affected absolutely on pregnant human female?

01 February 2018 5,938 7 View

I have a question about Detection of Brucella by PCR : no result on gel of product that is positive by real time pcr?

08 September 2017 1,344 3 View

What plasmid can I use to clone a 20 kb fragment in Brucella abortus?

08 September 2017 5,147 3 View

What plasmid can I use to clone a 20 kb fragment in Brucella abortus?

05 September 2017 4,717 3 View