Hi,
I have a question regarding the hematology analyzer for complete blood count for blood samples. I would need to analyze fixed blood samples and I would like to know if the analyzer could work also with this kind of samples. I know that the analyzer detects electric properties of the cells and with fixed samples I guess it won't be possible. Does anybody have experience with that? Thank you for your help!
I don"t have a direct answer but you can put it to the test. Run your sample (s), fixit and re-run it
Dear Mara Esposito in Peru I work in Forensic Sciences and we have arranged blood samples several times. We use commercial fixatives as well as alcohol-based solutions. We have tested the CBC of these samples and the results are not optimal, since the equipment (Sysmex) works with impedance and flow cytometry, we found many of the cellular parameters with values outside the limit and not very reliable. If you need any more questions, leave me a message at imbox. Regards!
Hi Mara
You may want to have a look at this paper. Good luck
Siva
doi: 10.1016/j.jim.2010.09.029. Epub 2010 Sep 27.. 2011 Jan 5;363(2):158-65.J Immunol Methods
Stability of immunophenotypic markers in fixed peripheral blood for extended analysis using flow cytometry
Cuc Davis 1, Xiaoying Wu, Weimin Li, Hongtao Fan, Manjula Reddy
Affiliations expand
- PMID: 20875824
- DOI: 10.1016/j.jim.2010.09.029
Abstract
The analysis of whole blood samples by flow cytometry for pharmacodynamic and biomarker assessments in clinical studies has been limited by the necessity to test these samples within a short time frame after blood collection. In most clinical studies, blood specimens are shipped to a centralized testing facility; it is critical to demonstrate specimen stability over a period of time which will encompass the time elapsed between specimen collection and testing. A possible solution to overcome this limitation is the use of a fixative to preserve the cell surface antigen stability in whole blood. We examined the stability of markers for T lymphocytes (CD3, CD4, CD8, CD45RA, and CD45RO), B lymphocytes (CD19), NK cells (CD16+CD56), activation (CD25 and HLA-DR), chemokine receptors (CCR5 and CXCR3) and skin homing (CLA) in fixed blood over 7 days and used this information to select the markers for global clinical studies. These assays with selected markers were further validated using fit-for-purpose approach (Lee et al., 2006) and to set the sample acceptability criteria for use in clinical sample testing. Most of the markers examined were stable when collected in Cyto-Chex® BCT for one week with the exception of the activation markers on T cells
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