Do I have to culture the cells in a solid matrix and make confucal image? Would some one provide me a protocol for this?
Use the single picture files and then HeliconSoft Helicon Focus to make the 3D picture.
19 January 2021 602 2 View
Hello Everyone, I know IL-1B is a soluble protein. So how to extract it from the supernatant? Please advice. Thanks!
05 January 2021 7,349 5 View
As of now, I am trying to explore the potential of using high/very high-resolution satellite imagery with machine learning (ML) to identify or map forest cover (e.g. rubber). As a newbie in this...
17 November 2020 7,187 3 View
Dear all, I have been testing a model that is in the Matlab library (https://es.mathworks.com/help/physmod/sps/ug/simplified-model-of-a-small-scale-micro-grid.html), this is named "power...
10 November 2020 2,117 3 View
I learned that in order to interpret the centrality indices, the CS coefficients resulted from a bootnet test are preferably above 0.5 and above 0.25 is acceptable. In my network, the CS...
29 October 2020 3,545 3 View
Univariate normality test tells me I only have 2 values among my predictor variables that are >3 SD. Multivariate normality test tells me a have a few outliers. I also plotted residuals using...
27 October 2020 9,579 5 View
I've been working on the network analysis of adolescent depressive symptoms. I've estimated the network, the centrality indices and network stability using packages qgraph and bootnet. Then I want...
22 October 2020 5,591 3 View
Some querries. A robust panel data analysis was done by my statistician in my study covering two periods: Panel A : 2008-2018 16 Oil companies and Panel B: 1988-2018 - Three Major Oil companies...
08 October 2020 940 3 View
25 September 2020 9,936 2 View
Assuming I don't standardize beta coefficients (this was recommended for dyadic analysis), does anyone know if grand mean centering my predictors will help me better interpret beta coefficients?
16 September 2020 7,154 2 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
02 March 2021 3,060 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.
28 February 2021 7,883 3 View