If I would like to have x gene deleted in cells expressing An as well as B and not in cells which are A+B- or B+A-. How can I achieve this using cre-flox driven knockout strategy? Is there any other strategy?
You flox x and find you are Cre, which is active in A+B+ cells only, easy said, but for most of the Cre lines to double-check with a reporter mouse first.
It is not easy to get the cre driver (unless u'r lucky) which can delete a gene in 2 different cell types. I would generate two lines crossing the flox mice for gene x with the respective cre and then cross the x gene deleted mouse in cell type A with x gene deleted mice in cell type B.
@Kay-Dietrich Wagner - theres is no gene known A+B+ cell type only. that's the problem. Anyway Thanks though!
@ Prameladevi Chinnasamy - This strategy would also delete x in A+B- and B+A- cells also. I am just looking for theoretical possibility now. Is it possible to express one domain of Cre in A+ cells and another domain of cre in B+ cells? I don't know if domains of cre expressed separately would be functional or not? But this would make sure expression of (assumed) functional cre in A+B+ cells only. And then have x gene floxed. Practically heroic experiment but is it feasible theoretically?
OK, so I had the same question. So for those of you who are still wondering: The technology was/is available. Awatramani et al (2003) used Cre and Flp https://www.nature.com/articles/ng1228.pdf
More recently, Plummer et al (2015) added a third recombinase, Dre http://dev.biologists.org/content/develop/142/24/4385.full.pdf
Even more recently, Karimova et al (2018) describe a 4 recombinase system https://www.nature.com/articles/s41598-018-32802-7
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