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Questions related from Kay-Dietrich Wagner
I tried to find information regarding the vector backbone of the Santa Cruz small hairpin vectors but until now without success. Is it a l'antiviral expression system or what is the promoter in...
17 May 2022 9,431 0 View
Did anybody try to passage mouse neonatal cardiomyocyte cultures? The cells are usually grow well. I just need to change the plate format from 6 well to 96 well for the Elisa reader. What is the...
28 January 2022 9,991 0 View
We used before the Promega reporter lysis buffer and a freeze-thaw cycle to measure Luciferase in suspension cells. Does anybody know a protocol/lysis buffer that works without centrifugation and...
17 February 2020 8,582 2 View
I am looking for a good surgical monitoring system for mice, mainly ECG recording. For different suppliers it is mostly the same Scintica Rodent Surgical Monitor+ system. Does anyone know a...
01 March 2019 2,242 1 View
Has anybody an idea what might be in the Vector M.O.M. blocking reagent? The kit works very well in background reduction and one could use rabbit antibodies on rabbit etc. if we would know the...
03 December 2018 7,682 3 View
I'm looking for a reliable cell culture model to test potential cardiotoxicity of a large number of compounds.
27 March 2018 1,898 4 View
We isolated primary endothelial cells from Tamoxifen-inducible Cre mice crossed to floxed animals. What would be a good concentration and timing to induce recombination in vitro?
10 March 2017 4,342 2 View
I want to establish stable clones from K562 cells in suspension culture. What is the best method for transfection except electroporation?
07 October 2016 3,469 3 View
In our common cell culture facility people always put bleach in the waste bottles. It stays there for weeks - thus I assume it has not much activity, but we inhale plenty of chlorine gas. Suggestions?
30 August 2016 4,829 3 View
