Which sequence could induce higher immunogenicity, S polypeptide or only S1? and how to isolate S1 from a vector containing the whole seq. of S?

I have a clone of DNA sequence encoding the full spike protein (S1+S2)

01 January 2021 7,821 1 View

Why RNA is totally degraded?

18 October 2020 5,252 22 View

How to prepare 37% Formaldehyde from paraformaldehyde?

I want to prepare 37% formaldehyde for denatured formaldehyde agarose gel preparation (for RNA) How can I prepare 37% formaldehyde? And how long this solution would be stable?

11 October 2020 914 4 View

What is the concentration of saturated solution of bromophenol blue?

I want to know the concentration of a home-made saturated aqueous solution of Bromophenol blue. How could I know when I add unknown number of grams of the dye?

05 October 2020 3,423 3 View

What is a good pretext task for self supervised lane detection in autonomous driving?

I have been looking into self-supervised methods in computer vision. For example Digging Into Self-Supervised Monocular Depth Estimation which looks at three consecutive frames of video, tasks a...

23 July 2020 8,232 1 View

What are some ways (aside from: a. mouse weight b. girth) to measure tumor growth in mice?

We have mice injected w/ specific cancer cell lines. The cancer cell lines failed to pick up the luciferase bioluminescent tag. We have a rubric of categories to measure the mice, but I was...

25 March 2020 5,991 2 View

Can anyone help me to find the psychometric characteristics for the JCQ VERSION 2 developed by Karasek??

I am using Job demands & control model in my thesis, and I want to use JCQ 2 questionnaire, but i cant find the psychometric characteristics

08 February 2020 2,240 3 View

Can we use a systemic NOAEL of a chemical product in a oral-administration effects study ?

Good evening, I have a already esablished Overall Systemic NOAEL (mg/Kg bw/day) for a pesticide wich i'll be studing the effect by oral administration on rats, can i use directly this dose in my...

08 November 2019 8,265 1 View

References about GA convergence or non-convergence?

I am looking for references to show that GA solutions do not necessarily converge to optimal solution to defend the use of an integer program, exact solution. I want to criticize heuristic and...

05 August 2019 900 5 View

In Data Envelopment Analysis (DEA), what to do if you are comparing with respect to outputs only?

Can we do that? And if yes, assuming that we have negative and positive output features, how to deal with them? By negative and positive features I do not mean value, but a feature like happy and...

22 January 2019 2,068 3 View

No title

28 February 2021 9,936 2 View

Can I get information about research laboratories that are currently having in culture, internationally/locally standardised microorganisms?

Microorganisms of interest include; E.coli; Salmonella typhimurium; Pseudomonas aeroginosa; Streptoccocus pneumoniae; Staphyloccocus aureus and Candida albicans. Locations closer to Port...

18 February 2021 6,500 3 View

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

What are the main reasons to grow Escherichia coli O157:H7 and Salmonella Typhimurium in food products under refrigeration storage 4 C?

24 January 2021 9,987 3 View

What is the best way to isolate multiple Salmonella serovars from a single sample?

I am trying to isolate multiple Salmonella serovars from a single food sample. I started by incubating the sample in a non-selective BPW enrichment and from there used multiple selective...

19 January 2021 8,365 4 View

I need help in peptide oxidation during deprotection group removal?

Hey! I need help on peptide (22 aa) oxidation.This peptide has no methionine, 1 tryptophan & 1 tyrosine. also has 4 cysteine so I used 2 protection groups (trt & acm). For ACM removal, I...

03 December 2020 3,225 2 View

What are the antimicrobial categories and agents that are currently considered to define a MDR, XDR and PDR Salmonella?

What antimicrobial categories and agents are currently considered to define non-typhoid Salmonella as multidrug resistant (MDR), extremely / extensively drug resistant (XDR) or pandrug resistant...

19 November 2020 755 3 View

How to knock out the gene of multidrug-resistant salmonella typhimurium?

During the knockout process, I found that my Salmonella was resistant to multiple antibiotics and could not screen successfully imported transgenes. Moreover, my bacteria seems to be very...

12 September 2020 6,683 1 View

Determination of Lipid A in Salmonella?

Im working with a S. typhimurium strain. Until now when i wanted to have a look at the amount of Lipid A present in the cells, i did silverstainings with proteinaseK treated whole cell samples....

26 August 2020 9,455 1 View

Is neccesary to add methionine after BamH I cloning site in a pRSET A vector in orther to make the expression work?

Hello, I am trying to express a fusion protein using a pRSET A vector in C41(DE3) E. coli strains. I have tried many ways and I am trying to see if my sequence is correct. My question is if it is...

13 January 2020 7,901 2 View

Has anyone detected amino acids using Western blots?

Hi all, I am trying to find a method to measure amino acids such as methionine and homocystiene in a fluid. We have used dot blots in the past but I am wondering if anyone has successfully used...

31 December 2019 1,883 3 View