Which sequence could induce higher immunogenicity, S polypeptide or only S1? and how to isolate S1 from a vector containing the whole seq. of S?

I have a clone of DNA sequence encoding the full spike protein (S1+S2)

01 January 2021 7,821 1 View

Why RNA is totally degraded?

18 October 2020 5,252 22 View

How to prepare 37% Formaldehyde from paraformaldehyde?

I want to prepare 37% formaldehyde for denatured formaldehyde agarose gel preparation (for RNA) How can I prepare 37% formaldehyde? And how long this solution would be stable?

11 October 2020 914 4 View

What is the concentration of saturated solution of bromophenol blue?

I want to know the concentration of a home-made saturated aqueous solution of Bromophenol blue. How could I know when I add unknown number of grams of the dye?

05 October 2020 3,423 3 View

What is a good pretext task for self supervised lane detection in autonomous driving?

I have been looking into self-supervised methods in computer vision. For example Digging Into Self-Supervised Monocular Depth Estimation which looks at three consecutive frames of video, tasks a...

23 July 2020 8,232 1 View

What are some ways (aside from: a. mouse weight b. girth) to measure tumor growth in mice?

We have mice injected w/ specific cancer cell lines. The cancer cell lines failed to pick up the luciferase bioluminescent tag. We have a rubric of categories to measure the mice, but I was...

25 March 2020 5,991 2 View

How to design load curve to take measurements for solar heater through the day?

I make measurements for the master thesis of solar heater. I need to design a load curve to obtain measurements for natural consumption of people. I will discharge solar heater tank through the...

29 February 2020 1,413 4 View

Can anyone help me to find the psychometric characteristics for the JCQ VERSION 2 developed by Karasek??

I am using Job demands & control model in my thesis, and I want to use JCQ 2 questionnaire, but i cant find the psychometric characteristics

08 February 2020 2,240 3 View

Can we use a systemic NOAEL of a chemical product in a oral-administration effects study ?

Good evening, I have a already esablished Overall Systemic NOAEL (mg/Kg bw/day) for a pesticide wich i'll be studing the effect by oral administration on rats, can i use directly this dose in my...

08 November 2019 8,265 1 View

References about GA convergence or non-convergence?

I am looking for references to show that GA solutions do not necessarily converge to optimal solution to defend the use of an integer program, exact solution. I want to criticize heuristic and...

05 August 2019 900 5 View

Where can I obtain Streptomyces lividans strains for enzyme production?

I am having trouble figuring out where to obtain Streptomyces lividans strains that are commonly used for enzyme production. Specifically I am searching for the following strains: S. lividans...

25 May 2020 6,205 3 View

Is it normal that Streptomyces fradiae culture is clumpy/particles/precipitated?

Hello, I cultured Streptomyces fradiae from a freeze-dried sample following ATCC protocol: use 6ml broth (tryptone 5g/l, yeast extract 3g/l) to rehydrate the sample and culture at 26 degrees...

05 February 2020 1,232 3 View

I need to extract secondary metabolites from Actinomycetes, can anyone help me ?

Hi, i'm workint now on Actinobacteria (Streptomyces) cultured on ISP2, and i need to extract it's metabolites to reveal its insecticidal effects, can anyone guide me which protocolor or procedure...

01 February 2020 8,450 3 View

Is it normal that Streptomyces fradiae culture is clumpy/particles/precipitated?

Hello, I cultured Streptomyces fradiae from a freeze-dried sample following ATCC protocol: use 6ml broth (tryptone 5g/l, yeast extract 3g/l) to rehydrate the sample and culture at 26 degrees...

01 February 2020 8,399 3 View

Could anyone help me to identify which is mouse crypt of this picture below?

11 December 2019 6,115 3 View

Which one is better citrate buffer or Na-phosphate buffer?

I want to work with Streptomyces sp as whole organism and its crude LPMO (lytic polysaccharide Monooxygenase) for sugarcane bagasse pretreatment for bioethanol. Which one is better to use and...

09 October 2019 6,174 3 View

GDNA stability at 55 degrees?

I was extracting genomic DNA from Streptomyces the previous day and left my gDNA in the oven (55 degrees Celsius) to dry at the very last step. I had to rush off for a seminar and completely...

11 September 2019 9,441 2 View

GDNA stability at 55 degrees?

I was extracting genomic DNA from Streptomyces the previous day and left my gDNA in the oven (55 degrees Celsius) to dry at the very last step. I had to rush off for a seminar and completely...

08 September 2019 7,719 2 View

Using 5-FC for plasmid curing - confirmation steps?

To cure my Streptomyces strain of a plasmid, I used 5-FC as a negative selection marker. So, cells without the plasmid (which are the ones I'm looking for) will be able to grow on agar plates...

17 July 2019 5,854 2 View

Using 5-FC for plasmid curing - confirmation steps?

To cure my Streptomyces strain of a plasmid, I used 5-FC as a negative selection marker. So, cells without the plasmid (which are the ones I'm looking for) will be able to grow on agar plates...

06 July 2019 5,350 2 View