Sameh Fayez

7 Questions 5 Answers 0 Followers

Questions related from Sameh Fayez

Does anyone have advice on how to remove starch or carbohydrates from protein samples?

i am trying to apply Buiret method for determining the protein content using spectrometer. it is a very accurate method but very sensitive, presence of carbohydrate and starch especially interfere...

19 October 2016 8,513 5 View

Does anyone have suggestion for the house keeping gene to be used in qRT-PCR of bacteria, Salmonella?

working on heat shock genes and try to make a blank for total genomic transcription

17 August 2016 2,734 3 View

I am searching for a program which can make image analysis of the protein profiles?

SDS-PAGE of stress test (heat shock) for salmonella cholerosius

21 June 2016 916 2 View

What is the best way to extract protein form bacterial cells?

I am working on heat shock protein, looking for best protocol for extracting and isolating protein of bacterial cells

23 April 2016 2,179 11 View

How can I get rid of bacterial intracellular methionine pools (Salmonella) ?

How can I get rid of bacterial intracellular methionine pools (Salmonella) ? is there a specific protocol?

14 April 2016 9,984 0 View

If I want to capture the RNA of specific stress proteins (heat shock), at what time should I do that after inoculating bacteria in browth?

bacteria has phases in growth. but there is a perfect time in which some balance between exhausting feeding material, gene expression and excreting toxic excretions. So, there must be a perfect...

12 February 2016 8,833 1 View

Hello colleagues.how can I isolate the RNA without being degraded assuming that the half life time of these fast respose genes RNA is little minutes?

I am studying the thermotolerance of a strain of salmonella that has been proved the ability to survive after exposing to temperature of 80C For 10 minutes and this strange trait for salmonella...

10 February 2016 8,565 2 View