How to calculate MDA content from absorbance values at 532 and 600 nm?

in analysing MDA content in plant tissues, I took 0.2 g leaf tissue (fresh welght), added 5 ml 0.1% TCA and samples were grind. samples then were centrifuged at 12500 rpm for 20 minutes. 3 ml from...

16 July 2019 6,884 2 View

How can I store stool specimen for Molecular diagnosis for human parasites Is there a difference of storing for PCR or real PCR or multiplex ?

Molecular stool

08 January 2019 8,186 3 View

How you calculate the sample size for project on a clinical disease?

How you calculate the sample size for project on a clinical disease?

21 July 2018 9,389 9 View

Can somebody tell me the most common microorganism isolated from ICU?

Can somebody tell me the most common microorganism isolated from ICU?

23 July 2017 9,542 5 View

Why some planes in orthorhombic structure have four miller indices?

we know the orthorhombic structure has three axes and hexagonal structure has four axes. therefore, the miller indices for orthorhombic structure and hexagonal structure are (hki) ,(hkil)...

13 February 2017 5,956 3 View

Why we must modify pH value in sol-gel method ?

23 May 2016 906 9 View

Does anyone know some papers that studied thermal analysis (TGA-DTA) and (FTIR) for CaBi2Nb2O9 ceramic?

I want to these paper please .

16 October 2015 9,480 2 View

How do I test normality for two independent groups?

Hi, I am doing comparison between two independent groups; patient group of 24 subjects and control group of 34 subjects. My questions are: 1) Is testing normality relative? I mean is it sufficient...

07 October 2015 5,646 21 View

How can I correct results for a substance measured in two different machines?

10 June 2015 927 6 View

What do you consider the diversification and intensification strategies in GAs?

Hello, What do you consider the diversification operator in GAs? is it the crossover or the mutation operator? I always thought it is the crossover because it provides large jumps in the solution...

13 March 2015 9,451 1 View

No title

02 March 2021 3,060 3 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View