I would like to recover viruses from ground water to do metagenomic sequencing.
From my reading I found the researchers used cellulose nitrate filter membranes. In Egypt the available filter membrane is polycarbonate membrane filters, is there is anyone tried before polycarbonate? and what are the results after DNA extraction?
Also, are there other methods for virus recovery from water?
Not me but others have. See here https://web.pdx.edu/~kstedman/PCTE_virus_counting_protocol.pdf
I will suggest Anodisks or probably better as you can grind them with the sample, they are brittle and expensive...
Dear Ms. Mattar,
you could try with reverse osmosis or nanofiltration.
this article is related with your research field. You could take a look.
https://www.mdpi.com/2077-0375/9/12/160
regards,
Uros
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