I tried extracting DNA from yeast cells but the sample couldn't show bands on the gel. Can anyone suggest a best protocol to isolate DNA from yeast?
HI, there are a lot of kits for DNA extraction from yeast cells. Ask the companies for free samples.
http://www.epibio.com/applications/nucleic-acid-purification-extraction-kits/dna-purification-genomic/masterpure-yeast-dna-purification-kit
http://www.piercenet.com/product/yeast-dna-extraction-kit
http://www.zymoresearch.com/yeast
....
Cheers, Nadine
Hi there,
For what purpose do you need this DNA? If it's just to use it as a template for PCR, you don't need a lot.
Hi, i can recommend this page for all kind of methods working with yeast. For standard protocols you should follow the first two links on the page. http://wiki.yeastgenome.org/index.php/Methods
Have fun!
If you are just doing endpoint PCR you can heat the cells in 0.02 M NaOH for 10 minutes.
For qPCR we wash with water, treat with zymolyase, then incubate at 95 C for 5 minutes to lyse. The lysates go directly into the qPCR mastermix.
Thank you ... I ll give it a try
Do use a specific kit. Is the best and easy.
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