Phytic acid limits bioavailability of micronutrients: Is challenge for biofortification?

As many researcher have confirmed that phytic acid content basically present in cereals and legumes crop that responsible for limiting bioavailability of micronutrients (Fe and Zn) in seeds. Lets...

31 January 2021 5,556 8 View

Does it mandatory to show all the synthesis with the same procedure in review article...?

When we are writing a review article if I want to add the previous synthesis of a particular molecule...but there are so many papers with the same procedure on that molecule...does it mandatory to...

18 November 2020 1,109 4 View

Biofilm Biofertilizers- New Generation biofertilizers?

Biofilm Biofertilizers-It is being applied to adhere the surface of soil and plant matrix containing consortia of various micro-organism that cut down chemical fertilizer. It is cost effective...

12 October 2020 4,883 13 View

Ethics in research publications?

In present scenario, lots of work were done by researchers and no doubt some of these have potential to change mindset of others that visible through benefiting farmers and other stakeholders....

09 October 2020 4,087 7 View

What input parameters are need to be provided while Validation of results of Experimental Tension test in ANSYS Workbench?

I have created new material in ANSYS Workbench with parameters obtained from the experimental tension test. I have provided the data of Stress strain, E, Syt, Sut, density, poissions ratio while...

13 August 2020 5,458 5 View

Future of cadaveric dissection in anatomy teaching after COVID Pandemic?

Cadaver dissection are the best adopted and accepted teaching tool in anatomy teaching from the accident time till date. Does the dissecting room still have a place in educating our under- and...

02 August 2020 2,948 17 View

Will my revised and resubmitted paper be regarded as a new submission?

The editor commented that the manuscript in the current format is unsuitable for acceptance. However, the editor recommend to revise the manuscript in accordance with the reviewers comment and...

30 July 2020 6,272 4 View

How solar energy is useful in agricultural practices?

i want to study share of renewable energy in agricultural practices especially in developing countries. pls suggest any research paper or work related to this topic.

05 March 2020 7,051 34 View

How to model slab section for pushover analysis in SAP2000 ?

I am performing pushover analysis on a RCC building in SAP2000. Whenevr I model Slabs as thin-shells, beams connected to them exhibit a brittle behaviour and fail directly after the yield point....

29 April 2019 4,162 5 View

How many hypotheses should I write for my study?

For my experimental study I created a course material for the visually challenged and implemented it by designing a learning model. Both are unique. Should I write two hypotheses, one for the...

25 March 2019 7,687 6 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View