What are some good polymer to use for packaging a biological product ,which can be steril by gamma?

As different polymers with different characteristics are exist,I want to know What polymers can be used for packaging an orthobiologic product(bone allograft),which can have enough resistent to...

22 December 2020 8,196 5 View

For LDH activity, is there any difference to add NADH, pyruvate and Tris separately in the well or prepare a mixture of them and then add?

Hi everybody, For LDH enzyme activity, I prepared to different series of buffers based on two protocols: 1-I prepared the solutions of 100 mM Tris, 3.6 mM Sodium pyruvate, 3.4 mM NADH...

22 October 2020 9,855 3 View

Nursing article about corona virus?

Hello Dear I need to catch scholarly articles about people attitude about contagious diseases like corona virus and how to mange their fear despite of maintaining their sensation about it

23 March 2020 6,936 12 View

Thin films of tio2 is made with sol gel (dip-coating) I want to know why when thin film thickness of Tio2 increases, anatase change to brookite?

Thin films of TiO2 is made with sol gel (dip-coating) I want to know why when thin film thickness of TiO2 increases, anatase change to brookite?

26 April 2018 2,142 4 View

Can anybody help me about removing of endotoxin from bacteriophage?

Hi. How can I remove endotoxin from bacteriophage lysate with a simple and high-efficiency method?I will appreciate to help me.

17 October 2016 4,544 1 View

How can I get the clear and high-quality image of my cells have been transformed by GFP vector under florescent microscope?

06 September 2016 6,924 4 View

When results of otsu method is better?for more levels or lower levels?

17 June 2016 1,571 3 View

Hybridoma cell cultures with HT medium?

Dear my colleages I am culturing hybridoma cells about 3 weeks. Now They are in the T-flask. I don't know how many times I must culture them in HT Medium?

27 February 2016 5,245 3 View

Which thresholding techniques are better if our image has Intensity homogeneities?

27 October 2015 9,872 17 View

I am looking for SAS software code for Augmented Design. Can someone help me out?

30 January 2015 2,107 18 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View