What is the unit of extinction coefficient?

Hi dear researchers Is it right use 1/mmol unit for extinction coefficient (molar absorption coefficient) instead of 1/mM? (I see this in some atticles)

11 December 2018 3,989 4 View

Why DNA polymerase require a primer but RNA polymerase does not?

Dear researchers Why does DNA polymerase need an RNA primer? but RNA polymerase don't? What happened in catalytic site?

05 June 2018 4,919 0 View

What is the role of polyubiquitin in DNA repair?

Dear researchers I want to know polyubiquitin roles in DNA repair .(briefly)

04 May 2018 8,492 0 View

What is metagenome?

Hi dear researchers I Want to know about metagenome and metataxonomic (definitions and applications). Why we need metagenomics? How metagenomics differ from genomics?

03 April 2018 3,299 0 View

How many torsion angles(Dihedral angle) are there in nucleotide?

Dear researchers, I want to know about torsion angles in nucleotide. Can someone help me?

31 December 2017 2,982 0 View

Does H2O2 have oxidation effects on the nanomaterials?

Dear researchers, Nanomaterials such as nanotubes and nanoporouses are used for immobilization of peroxidase enzymes. H2O2 is one of the Horseradish peroxidase enzyme substrates. Does oxidation...

31 December 2017 2,653 0 View

How Recombinant Insulin is produced?

Dear researchers, I want to know about methods of producing recombinant insulin. Could anyone help me with some literature. Thanks in advance

11 December 2017 2,956 4 View

How sulfide concentration is measured by standard spectrophotometric method?

Hi dear researchers In a nature report "Amperometric inhibitive biosensor based on horseradish peroxidasenanoporous gold for sulfide determination" measuring sulfide concentration in a wastewater...

10 November 2017 5,111 3 View

How gene expression can be regulated by mRNA structure?

Hi dear researchers According to this sentence "mRNA Spatial shape can effect on gene expression even the amount of gene expression" I waned to know how gene expression can be regulated by mRNA...

09 October 2017 3,209 4 View

How biological element attach to surface in biosensors?

I study a bout biosensors and bioreceptors... I am new biochemistry student...and I want to know a bout surface attachment of the biological element in biosensors... it is mystery for me ...

09 October 2017 1,228 4 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View