Hi dear researchers
I Want to know about metagenome and metataxonomic (definitions and applications).
Why we need metagenomics?
How metagenomics differ from genomics?
Hi dear researchers Is it right use 1/mmol unit for extinction coefficient (molar absorption coefficient) instead of 1/mM? (I see this in some atticles)
11 December 2018 3,989 4 View
Dear researchers Why does DNA polymerase need an RNA primer? but RNA polymerase don't? What happened in catalytic site?
05 June 2018 4,919 0 View
Dear researchers I want to know polyubiquitin roles in DNA repair .(briefly)
04 May 2018 8,492 0 View
Dear researchers, I want to know about torsion angles in nucleotide. Can someone help me?
31 December 2017 2,982 0 View
Dear researchers, Nanomaterials such as nanotubes and nanoporouses are used for immobilization of peroxidase enzymes. H2O2 is one of the Horseradish peroxidase enzyme substrates. Does oxidation...
31 December 2017 2,653 0 View
Dear researchers, I want to know about methods of producing recombinant insulin. Could anyone help me with some literature. Thanks in advance
11 December 2017 2,956 4 View
Hi dear researchers In a nature report "Amperometric inhibitive biosensor based on horseradish peroxidasenanoporous gold for sulfide determination" measuring sulfide concentration in a wastewater...
10 November 2017 5,111 3 View
Hi dear researchers According to this sentence "mRNA Spatial shape can effect on gene expression even the amount of gene expression" I waned to know how gene expression can be regulated by mRNA...
09 October 2017 3,209 4 View
I study a bout biosensors and bioreceptors... I am new biochemistry student...and I want to know a bout surface attachment of the biological element in biosensors... it is mystery for me ...
09 October 2017 1,228 4 View
Hi dears How do nanoparticles interact with enzymes after immobilization? How can bioinformatics tools be used for this? can I use bioinformatics free softwares with simple system to know...
01 January 1970 247 7 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
02 March 2021 3,584 1 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...
01 March 2021 2,512 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View