Hi Riaz!
You may wish to look at this paper, describing among other things reasonable procedures for callose extraction and a fluorometric procedure for quantitative callose analysis.
Plant Physiol. (1985) 77, 544-551: Chitosan-Elicited Callose Synthesis in Soybean Cells as a Ca2+-Dependent Process'
by: HARALD KOHLE2, WOLFGANG JEBLICK, FRAUKE POTEN, WOLFGANG BLASCHEK, AND HEINRICH KAUSS
Good luck -- Joh. W.
Dear Riaz Muhammad
Pls. find the attached files
https://www.ncbi.nlm.nih.gov/pubmed/17126734
http://onlinelibrary.wiley.com/doi/10.1111/j.1467-7652.2010.00555.x/full
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4457719/
http://www.bio-protocol.org/e1429
https://academic.oup.com/aob/article/116/4/613/93389/Zinc-induces-distinct-changes-in-the-metabolism-of
http://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-2779-y
http://www.nature.com/articles/srep14675
https://academic.oup.com/aob/article/84/6/715/2588105/Explant-Orientation-and-Polarity-Determine-the
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0073742
https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-2663-9
http://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-9-127
http://link.springer.com/article/10.1023/A%3A1021555809036?no-access=true
https://www.researchgate.net/profile/Elke_Cardoso/publication/226608764_Manganese_toxicity_and_callose_deposition_in_leaves_are_attenuated_in_mycorrhizal_soybean/links/54f9ef380cf29a9fbd7c58db.pdf
http://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-9-127
http://www.plantcell.org/content/13/4/755.full
https://academic.oup.com/jxb/article/63/7/2717/516205/Phenotypic-plasticity-and-water-flux-rates-of
Hoping this will be helpful
Regards
Prof. Houda Kawas
Article Manganese toxicity and callose deposition in leaves are atte...
Hi Riaz
I think you can see this paper "Reference"
https://www.cell.com/developmental-cell/pdf/S1534-5807(15)00109-4.pdf
@Laith Al-Ani @Johannes Wostemeyer @Houda Kawas, Thanks everybody for sharing articles, I have read these articles, But i didn't find Calculation for callose, as i want to measure quantitative callose by florescent spectrometer. Please help me how i can calculate callose quantitatively.
Dear Riaz Muhammad
Pls. find the attached files
http://onlinelibrary.wiley.com/doi/10.1111/j.1467-7652.2010.00555.x/full
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3772824/
https://academic.oup.com/jxb/article/61/2/483/619197/Differential-gene-expression-analysis-provides-new
http://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-2779-y
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4457719/
https://www.ncbi.nlm.nih.gov/pubmed/17126734
http://onlinelibrary.wiley.com/doi/10.1111/j.1467-7652.2010.00555.x/full
http://www.plantphysiol.org/content/162/1/9.full
https://www.jstor.org/stable/4269179?seq=1#page_scan_tab_contents
https://www.jstor.org/stable/23378418?seq=1#page_scan_tab_contents
http://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-9-27
https://www.ncbi.nlm.nih.gov/pubmed/15008821
http://www.nature.com/ni/journal/v16/n4/fig_tab/ni.3124_SF2.html
http://www.chromedia.org/chromedia?waxtrapp=mkqjtbEsHonOvmOlIEcCArB&subNav=cczbdbEsHonOvmOlIEcCArBP
http://www.nature.com/ni/journal/v16/n4/full/ni.3124.html
http://www.jstor.org/stable/43215233?seq=1#page_scan_tab_contents
http://link.springer.com/article/10.1007/BF00403034
Hoping this will be helpful, good luck
Regards
Prof. Houda Kawas
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