What is the importance of miRNA threshold? Are miRNAs threshold have anything to do with protein expression level?

Iam working with miRNAs and in our lab we detect different miRNAs threeshould in different cell lines and it seems like it is vary and these variations has nothing to do with protein expression...

09 October 2015 6,998 4 View

How I can quantify protein expression?

09 October 2015 713 7 View

Can any one help me to find a referance to this photo???

31 December 2014 6,370 7 View

Are there any miRNAs that control phosphorylation of DNA proteins?

10 November 2014 9,337 3 View

Does metastasis represent an advantage or disadvantage during animal model study?

I am willing to do in vivo study (Xenograft) using 4T1 cell line (murine breast cancer cell line) and as I understand this cell line can highly metastasize to different organs. My goal of the...

08 September 2014 8,872 8 View

How can I calculate the concentration of 50nM miRNA 100?

07 August 2014 6,063 8 View

I want to run overnight western blot electrotransfer, what voltage should I use? The protein is p-ATM.

I always blot overnight 10 volt and then 1hr - 100 volt but the signal is always so weak ... any ideas?

06 July 2014 1,824 15 View

How can I carry out the MTT test with HCC1599?

I want to measure growth inhibition using HCC1599, but as it is a suspension cell line, I need to remove the medium before I add the MTT solvent without losing cells.

04 May 2014 9,165 5 View

Is it reasonable to compare Luminal A, Luminal B to triple negative breast cancer cell lines?

I am working in breast cancer research, comparing Luminal A, Luminal B to TNBC cell lines. I see that some of the Luminals share characteristics of TNBC. I wonder if we should compare all Luminals...

03 April 2014 236 11 View

How can I subculture HCC1599 cell line?

I tried to follow the protocol of DSMZ and it didn't work. At the end, the cells were contaminated and I couldn't run my experiment until now. Does anyone have experience with this cell line?

09 October 2013 6,987 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View