Hamed Abdelazim Helal

12 Questions 40 Answers 0 Followers

Questions related from Hamed Abdelazim Helal

How I can quantify protein expression?

Iam trying to detect proteins expression (ATM, Rad51, DNA-PKcs ... etc) in my Ph.D. Iam using western Blot right now but Iam looking to use more precise method. Can any one suggest other methods?

10 October 2015 794 7 View

What is the importance of miRNA threshold? Are miRNAs threshold have anything to do with protein expression level?

Iam working with miRNAs and in our lab we detect different miRNAs threeshould in different cell lines and it seems like it is vary and these variations has nothing to do with protein expression...

10 October 2015 7,075 4 View

Can any one help me to find a referance to this photo???

this A-T cells (fibroblasts) sensitivity after ionizing radiation

01 January 2015 6,445 7 View

Are there any miRNAs that control phosphorylation of DNA proteins?

I want to know if the miRNA which block the protein translation is also block protein phosphorylation or this is not the case?

11 November 2014 9,409 3 View

Does metastasis represent an advantage or disadvantage during animal model study?

I am willing to do in vivo study (Xenograft) using 4T1 cell line (murine breast cancer cell line) and as I understand this cell line can highly metastasize to different organs. My goal of the...

09 September 2014 8,947 8 View

How can I calculate the concentration of 50nM miRNA 100?

I am using MDA-MB-231 cell line in 6 well plate (300.000 cell/well) and I want to transfect them with 50nM and all the information I had is the mount is 5nmol. I am using RNAiMax.

08 August 2014 6,145 8 View

I want to run overnight western blot electrotransfer, what voltage should I use? The protein is p-ATM.

I always blot overnight 10 volt and then 1hr - 100 volt but the signal is always so weak ... any ideas?

07 July 2014 1,906 15 View

How can I detect immunofluorescence foci in suspension cell lines?

I want to detect Rad51 foci with HCC1599 (Suspension cell) and I do not know if this is possible or not. The difficulty is that normally the cells we used are adherent and I do not have experience...

06 June 2014 7,358 3 View

How can I carry out the MTT test with HCC1599?

I want to measure growth inhibition using HCC1599, but as it is a suspension cell line, I need to remove the medium before I add the MTT solvent without losing cells.

05 May 2014 9,235 5 View

Is it reasonable to compare Luminal A, Luminal B to triple negative breast cancer cell lines?

I am working in breast cancer research, comparing Luminal A, Luminal B to TNBC cell lines. I see that some of the Luminals share characteristics of TNBC. I wonder if we should compare all Luminals...

04 April 2014 316 11 View

How can I subculture HCC1599 cell line?

I tried to follow the protocol of DSMZ and it didn't work. At the end, the cells were contaminated and I couldn't run my experiment until now. Does anyone have experience with this cell line?

10 October 2013 7,056 2 View

Are there different times to enter S phase in tumor cells?

I'm working with mamma cells, some of which grow slowly and others grow fast. I work mainly on the S phase, so I have to prepare my cells one day (24 hours) before the experiment and then run the...

08 August 2013 10,090 5 View