How can I describe the morphology of cell lunes?

Ivan Ricardo Vega Valdez @Ivan-Vega-Valdez

16 June 2020 0 2K Report

I want to describe te morphology change in treated cell lines, ¿what is the Best way or there is any manual or guidelines to describe it?

Badges
Science topic

More Ivan Ricardo Vega Valdez's questions See All

How can, granulometry or grain size data, be used in enrichment factor calculations?

As a stable element

23 July 2024 4,187 1 View

Is there anything faster than Xarray or Pandas out there?

Hello, dear RG community. Personally, I have found Xarray to be excruciatingly slow, especially for big datasets and nonstandard operations (like a custom filtering function). The only suggestion...

15 July 2024 4,705 2 View

O impacto da Gestão Estratégica de Marketing, na tomada de decisões empresariais?

o impacto da Gestão Estratégica de Marketing, na tomada de decisões empresariais?

10 July 2024 3,430 0 View

To what extent should "reading comprehension" be analyzed in an A.R. based on phonological awareness instruction to EFL students using a webtool?

Dear Colleagues and academy, I am conducting an action research study to understand the impact of phonological awareness activities using Quizlet and Boom on the reading comprehension skills of...

02 July 2024 8,480 0 View

My hippocampal mouse primary neurons clump when I plate them on microfluidic devices for axonal isolation. Any tip?

Hi all, I am working on both primary hippocampal mouse neurons and i3Neurons to isolate axonal material. I plate them in microfluidic devices in house made (like any other you can find on the...

01 July 2024 8,070 0 View

Can anyone recommend an antibody development course?

I would like to find a short course focused on the development of monoclonal antibodies covering the entire process, from the selection of antigen and carrier, to the purification of the final...

27 June 2024 5,618 1 View

Is it possible to use Overhangs with large different Tm with NEB Builder HiFi DNA assembly?

Dear cloning community, Is it possible to use Overhangs with large Tm difference with NEB Builder HiFi DNA assembly? 5´ 26 bp Tm 57ºC 3´ 24 bp Tm 72ºC Unfortunately, extending the 5´ doesn't...

17 June 2024 8,552 2 View

Can you use MTT assay for tissue samples?

Can you use the MTT assay of cell viability for tissue samples derived from rats e.g. liver, muscle, heart, brain, or is this method reserved for cultured cells? Thank you

29 May 2024 6,389 2 View

What are the features and best library to predict and clasify people?

Hello everyone, I am currently exploring several options to give the collected data the greatest value possible. I have demographic data on older people, where I perform various memory and mood...

02 May 2024 876 6 View

¿Que hipotesis seria un trabajo de investigación sobre ansiedad, depresión, rendimiento académico y conducta sofial en aula de estudiantes univer?

La Ansiedad y depresión son un problema de salud mental y los universitar

21 April 2024 8,736 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View