I'm going to experiment with sepharose CL-4B gel to isolate drugs that are not enclosed in liposomes. I need to degas enough before using the sepharose gel, and most of the references seem to be done using a vacuum pump. We don't have this instrument in our laboratory, so is it okay to degas sepharose using bath type sonicator? Wouldn't it affect the structure of the sepharose gel? Or is there any other way to remove the air from the sepharose gel? Thank you.