Hi everyone, I have a question on RNA integrity of my stored tissue sample.

Briefly, I have extracted RNA using spin column method from tissue stored in minus 80 (The tissues were stored one month back in minus 80). I performed 1% agarose gel electrophoresis to confirm the integrity of RNA. I have seen some bands but don't know whether it is total RNA or RNA contaminated with DNA. Please find the enclosed images and help me.

In the first lane i used a 1kb DNA ladder (we don't have RNA ladder available readily)

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