We performed restriction digestion and to analyze the restriction pattern by and received a bad result. What may be the reason behind it
the blurred shape of the cut dna suggests there is too much salt in the sample. Try running half as much sample and an uncut sample for comparison. run the gel at a lower voltage and a longer time to minimise heating the gel which always produces poor results.
Doni Sri Lakshmi Angadala If you could provide more information about the experimental setup, then a proper solution can be suggested for you to troubleshoot.
Have you purified DNA before digestion? Purification is must to remove salts and other contaminants (i.e., enzymes used in earlier experiments) that can hinder the downstream processes like digestion, ligation, etc. If you have purified the DNA then there is problem with the restriction enzymes. You can increase the incubation time from double as suggested by the manufacturer to overnight and you can also increase the volume of enzyme 1.5X to 2X if their efficiency has decreased.
What results were you hoping to see? I'm not seeing any labels to know what is was you digested & which (if any) samples are your controls.
You'll also want to include a DNA ladder.
Try again and good luck!
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