David F. Barker

6 Questions 208 Answers 0 Followers

Questions related from David F. Barker

What is the best multi-sample device for preparing chromatin by sonication?

From many samples (say 12 to 24). Is it a Bioruptor or the Episonic 2000 (Epigentek) or some other instrument? Effective cooling and processing of the samples in closed containers are two...

25 June 2015 4,132 7 View

Has anyone used BGI Americas for an RNA-seq project and/or have a basis for comparison of different companies offering RNA-seq?

BGI Americas is offering an apparently attractive promotion for RNA-seq and Bioinformatics services. http://bgiamericas.com/rna-seq-promotion/ Any suggestions for what a buyer should beware of or...

16 October 2013 9,264 3 View

How uniform is the microbiome of commercially sourced mice?

In experiments designed to test the effect of diet/drugs etc. on the mouse microbiome, one could assume that the initial microbial content of each mouse of one commercially-sourced set of mice is...

14 March 2013 5,900 9 View

How small of a "fold-change" in gene expression can be reliably measured by RT-qPCR?

When using Sybr green or TaqMan qPCR assays to measure gene expression changes, can differences that are less than at least two-fold be taken as credible? What minimum fold-change or percent...

14 February 2013 413 43 View

For metagenomic "profiling" which is more useful, DGGE or TaqMan based qPCR?

To obtain a quick, but reasonably accurate metagenomic profile or fingerprint without any deep sequencing, I have seen the use of both 16S rRNA analyzed with denaturing gradient electrophoresis...

26 October 2012 777 15 View

What is the advantage of expressing an shRNA vs a microRNA primary transcript in a lentiviral vector?

Is there any particular advantage, other than the smaller insert size, to expressing an shRNA rather than a microRNA primary transcript when using a lentiviral delivery system?

10 September 2012 5,578 7 View