Which forward primer I should order?

I have been trying to design primers for microRNA expression. The image shows that there are 2 sets of forward primer (Tm calculated in two different manners) and one universal "reverse primer" as...

10 November 2018 5,216 0 View

Pros and cons regarding expression profiling of microRNA-SNP using breast cancer cell lines?

I have found a SNP in a microRNA gene from my association study and now want to do the expression analysis in breast cancer cell lines (sub-type specific). What are the factors should I consider...

04 May 2018 674 0 View

How can I design vector with miRNA variant (SNP) for expression analysis?

I have found a miR-SNP which is significantly associated with my breast cancer case-control cohort. Now I want to see its expression in different breast cancer cell lines to find out the best...

03 April 2018 5,145 4 View

How can I view .map & .ped files in command promt in Windows using PLINK?

I have got .bed .bim and .fam files from a database and need to see whats inside. But I don't know the command to do so. As well as I would like to view my own .ped and .map files in the command...

08 September 2017 8,758 0 View

In vitro assay to screen anti-arthritic property of drug compound?

09 October 2016 9,263 4 View

Which cell-cycle phase is the best for drug treatment?

I have used the drug as a broad term here. Specifically my focus is on those drugs which have antioxidant property. Is there really any time point at which cells will respond best to the...

07 August 2016 4,857 0 View

HUVEC starvation, time and with or without serum?

To get the best ROS generation (i am expecting 40-50% increase than that of control), how long my HUVEC should be starved and what would be the serum percentage? i am using EBM-2 (Lonza) without...

06 July 2016 9,227 5 View

HBSS, with or without Ca2+/Mg2+ for DCFDA assay?

06 July 2016 1,167 5 View

Why HUVEC is giving low amount of protein?

Trying to measure protein concentration for Lucigenin assay. But even culturing at more than 80% confluency, after centrifugation and sonication getting only 330ug/ml of protein. Hard to maintain...

05 June 2016 1,227 2 View

Why did TNF-alpha treated HUVEC not generating significant level of ROS ?

I am culturing HUVEC up to 80% confluency (EGM-2; Lonza), then let them serum starved (EBM-2; Lonza) for 24hr and treated with TNF-a (10ng/ml) for 30min. With DCFDA I didn't get that much of...

05 June 2016 1,975 6 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View