I am working with some dried extract residues from protein isolation. I want to see which proteins I loose there. The protein content of these is around 25% and dry matter 93.7%. I usually use 1 mL of 1x Treatment Buffer for 50 mg protein, but in this case I would need to use 200 mg of my sample to reach the necessary protein content (50 mg). How can I change the dilution to be able to solubilize my sample but have enough protein to see in the gel?
Thank you in advance,
Veronica
https://www.researchgate.net/post/How_to_increase_protein_concentration_by_changing_sample_to_sample_buffer_ratio
Hope it helps.
You can try not changing volume/mass ratio, but use 4x concentrated buffer.
Follow this link maybe helpful
https://www.thermofisher.com/content/dam/LifeTech/global/Forms/PDF/protein-gel-electrophoresis-technical-handbook.pdf
regards
I totally agree: use 4x treatment buffer, this worked with my samples as well - sometimes I had protein contents between 8-20% with a dm between 85-95% ;-)
Thank you everyone, I will then use the 4x treatment and hopefully it will work.
Best Wishes
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