PAST diversity software not running under macOS Catalina... any suggestions?

Hello all, After the update to macOS Catalina, PAST diversity software is no longer running... It's not compatible anymore with the new 64 bits platform... any suggestions? other than using...

13 November 2019 4,647 2 View

Normalization and Homogeneity - Parametric or Non-Parametric?

Hi everyone, I performed a Shapiro-Wilk Test to check the normality of my 60 samples (n=4). If just 5 of my samples are not normally distributed, can I still perform an ANOVA? I also performed a...

06 July 2019 8,112 6 View

Why Protein standards in SDS-PAGE are not aligned in molecular weight?

Hi everyone, I wanted to compare 2 protein standards (from the same company) to decide which one to use but after I found that the location of molecular weights of the two different standards does...

08 August 2018 6,562 5 View

Why Protein standards in SDS-PAGE are not aligned in molecular weight?

Hi everyone, I wanted to compare 2 protein standards (from the same company) to decide which one to use but after I found that the location of molecular weights of the two different standards does...

07 August 2018 7,753 7 View

How can I change Sample-Buffer dilution for SDS-PAGE for low protein products?

I am working with some dried extract residues from protein isolation. I want to see which proteins I loose there. The protein content of these is around 25% and dry matter 93.7%. I usually use 1...

04 May 2018 9,358 6 View

Could any Botanist help me to identify this Ipomoea species, please?

Habitat/Location: Salvador de Bahia, Brazil. The plant seems to have grown naturally in a semi-natural ecosystem/urban garden at the University campus. I think this is not I....

24 August 2017 1,313 23 View

Do you know any primary source (prior to 1900) containing detailed descriptions of psychoactive plants or their use by American indigenous peoples?

I am interested in works written in any european language (from the first arrival of europeans to America up to the 19th century), which can be useful as valuable primary historical sources for an...

08 August 2017 5,844 12 View

Exosome Quantification

24 May 2017 3,750 3 View

Can someone advise on the best way to remove a dye from a crude extract of enzymes?

I have a crude extract of enzymes produced by Solid State Fermentation, but the microorganism produces a black/ brown dye. I already tried to different methods to remove dye from extracts, like...

04 July 2013 4,688 2 View

Does anyone have experience with molecular characterization of a filamentous fungi?

We isolated one fungi from the soil, and from its morphology we concluded it must be a Cladosporium genera ( a filamentous fungi , a mold ). I've revised different methods, but I dont know what...

30 June 2013 9,629 9 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View