I made chitosan nanoparticles. I centrifuge and nanoparticlesill be on the supernatent. The concentration used 0.75mg/ml chitosan and 0.75 mg/ml TPP all formula 15 ml in acetate buffer 0.1 M.
Thanks Dr.mahmoud. I tried to use speed vac (for dryness) but unfortunately i prepared these nanoparticles in buffer and the salts of buffer ppt too with them and so the yield was bigger than normal even i dried blank of buffer against them.