How can I avoid contamination of Genomic DNA during RNA extraction?

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Danijela Markovic

After the RNA extraction used DNase to remove any residual DNADNA

Anna Kopf

I could recommend TURBO™ DNase from Ambion (Life technologies). I used it in a previous study and it worked very well.

Muhammad Ali

Thank you very much for your suggestions. However, I was particularly asking about the possible reasons of DNA contamination and how it could be avoided during extraction? Obviously degradation of DNA by DNase is a necessary step before cDNA synthesis.