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Questions related from Muhammad Ali
When I prepare NGM plate for the growth of C. elegans, the plates are clear. However, when I spread culture of E. coli OP50 on NGM plates and incubate at 37 C overnight, I observed crystal like...
11 November 2017 789 4 View
I am using 16S primers for qPCR. However I got different Ct values in may treated and control sample (30.08 and 28.85). Would it lead to considerable error in final results? In my opinion,...
07 July 2015 3,393 10 View
Is there any possible way to find out genomic DNA contamination/residues in cDNA by analyzing results of qPCR? Can we get any information related to genomic DNA by Ct value?
06 June 2015 793 4 View
Kindly explane importance of cDNA dilution before qPCR and how one can determine best dilution factor for cDNA samples? How it will effect results of qPCR? In other words, how much quantity of...
06 June 2015 1,507 22 View
I need some information about house keeping genes to be used as internal control particularly for Pseudomonas syringae. Kindly let me know which house keeping genes are good to study gene...
01 January 2015 5,662 4 View
It is observed that RNA samples are often contaminated with genomic DNA. How can this contamination be avoided? What are the possible causes for genomic DNA contamination during mRNA extraction.
01 January 2015 5,096 3 View
I have a genome sequence however, sequence is in microsoft word file and annotation is present in excel file. How can I convert/merge this information into genbank format. (sequence is not...
10 October 2014 6,725 6 View
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10 October 2014 9,524 15 View
1) The sequence data is in contigs form. 2) Please suggest windows based tools. 3) Important literature/source would also be appreciated.
09 September 2014 8,395 18 View
I need some good expression vectors for Pseudomonas syringae for over expression of proteins and for the construction of complementary strain. along with that, I also need the source/link from...
09 September 2014 5,428 4 View
Which techniques should be used to confirm that gene has been knocked out and why? What about DNA sequencing of gene of interest by flanking primers?
06 June 2014 1,154 4 View
After constructing a mutant bacterial strain, I have to construct complementary strain. How can I confirm expression of gene of interest in complementary strain? Which techniques can be used for...
06 June 2014 10,049 1 View
We have a genome of laboratory bacterial strain. However, genome is at draft level and it is composed of nearly 200 contigs. Is it possible to identify PGI? If yes, which tools/softwares are easy...
06 June 2014 2,016 3 View
If we have to express a protein with His Tag, where should we put ATG in the recombinant vector (Vector sequence ATG ---6X His-------EK recognition site-MCS)? 1) ATG ----- His His His His ------...
11 November 2013 1,977 4 View
