Which are experimentally proved Best house keeping genes to study gene expression in Pseudomonas syringae?

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Why crystal like structures are formed on NGM plates cultured with E. coli OP50?

When I prepare NGM plate for the growth of C. elegans, the plates are clear. However, when I spread culture of E. coli OP50 on NGM plates and incubate at 37 C overnight, I observed crystal like...

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Any advice on the difference in the Ct value of reference gene in treated and control samples?

I am using 16S primers for qPCR. However I got different Ct values in may treated and control sample (30.08 and 28.85). Would it lead to considerable error in final results? In my opinion,...

06 July 2015 3,326 10 View

Is it necessary to dilute cDNA before qPCR? How much dilution should be made? How to determine best dilution factor?

Kindly explane importance of cDNA dilution before qPCR and how one can determine best dilution factor for cDNA samples? How it will effect results of qPCR? In other words, how much quantity of...

05 June 2015 1,350 22 View

Is it possibile to determine genomic DNA contamination in cDNA sample on the basis of qPCR results?

Is there any possible way to find out genomic DNA contamination/residues in cDNA by analyzing results of qPCR? Can we get any information related to genomic DNA by Ct value?

05 June 2015 738 4 View

How can I avoid contamination of Genomic DNA during RNA extraction?

It is observed that RNA samples are often contaminated with genomic DNA. How can this contamination be avoided? What are the possible causes for genomic DNA contamination during mRNA extraction.

31 December 2014 5,040 3 View

We want to remove the gaps/errors of our laboratory genomes with some reference genomes. Which software would be the best option?

09 October 2014 9,468 15 View

How can one convert genome sequence files of microsoft office into genbank format?

I have a genome sequence however, sequence is in microsoft word file and annotation is present in excel file. How can I convert/merge this information into genbank format. (sequence is not...

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Which are the best expression vectors for Pseudomonas syringae?

I need some good expression vectors for Pseudomonas syringae for over expression of proteins and for the construction of complementary strain. along with that, I also need the source/link from...

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What software/database could be used for identification of pan genome/core genome of user's own sequence data?

1) The sequence data is in contigs form. 2) Please suggest windows based tools. 3) Important literature/source would also be appreciated.

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What techniques are best to confirm construction of complementary strain?

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What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

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03 March 2021 687 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

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How do I increase the yield of my PCR product?

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How to quantitative analize the data of TNBS assay for gelatin?

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Sample dilution for quantitative analysis using ELISA?

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02 March 2021 7,670 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

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02 March 2021 1,146 2 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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How to calculate the annealing temperature of a primer pair?

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How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

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Cheng Zhan

Probably you can refer to this article "RNA-seq Analysis Reveals That an ECF σ Factor, AcsS, Regulates Achromobactin Biosynthesis in Pseudomonas syringae pv. syringae B728a".

Muhammad Ali

thank you very much for your cooperation

Ana J. González

Dear Muhammad,

The article by Sarkar and Guttman covers this topic in P. syringae:

Evolution of the Core Genome of Pseudomonas syringae, a Highly Clonal, Endemic Plant Pathogen, in APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Apr. 2004, p. 1999-2012.

I usually use rpoD and gyrB genes, whereas I have had trouble at times with gltA (cts).

Ana

Dear Ana

thank you very much for your help